Journal article
Minor class splicing shapes the zebrafish transcriptome during development
S Markmiller, N Cloonan, RM Lardelli, K Doggett, MC Keightley, Y Boglev, AJ Trotter, AY Ng, SJ Wilkins, H Verkade, EA Ober, HA Field, SM Grimmond, GJ Lieschke, DYR Stainier, JK Heath
Proceedings of the National Academy of Sciences of the United States of America | Published : 2014
Abstract
Minor class or U12-type splicing is a highly conserved process required to remove a minute fraction of introns from human premRNAs. Defects in this splicing pathway have recently been linked to human disease, including a severe developmental disorder encompassing brain and skeletal abnormalities known as Taybi-Linder syndrome or microcephalic osteodysplastic primordial dwarfism 1, and a hereditary intestinal polyposis condition, Peutz-Jeghers syndrome. Although a key mechanism for regulating gene expression, the impact of impaired U12-type splicing on the transcriptome is unknown. Here, we describe a unique zebrafish mutant, caliban (clbn), with arrested development of the digestive organs c..
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Awarded by National Institute of General Medical Sciences
Funding Acknowledgements
We thank Gabriel Kolle and Ivonne Petermann for technical expertise with RNAseq; Minni Anko, Oliver Sieber, Anuratha Sakthianandeswaren, Chris Love, and Dmitri Mouradov for valuable scientific discussions; Tyler Alioto for providing a scan of the zebrafish Zv8 genome assembly for U12-type introns; Cameron Nowell for microscopy; Val Feakes for histology; Janna Taylor for graphics; and Dora McPhee, Kelly Turner, Mark Greer, Tyson Blanch, and Lysandra Richards for expert fish husbandry. This work was supported by the National Health and Medical Research Council of Australia [Project Grants 433614 and 1024878 (to J.K.H.) and 637395 (to G.J.L.), Program Grant 487922 (to J.K.H.), and Enabling Grant 455871], Australian Research Council Grant DK060322 (to G.J.L.), National Institutes of Health Grant DK060322 (to D.Y.R.S.), a Boehringer Ingelheim Fonds PhD fellowship and a University of Melbourne International Postgraduate Research Scholarship (to S. M.), the Australian Cancer Research Foundation, and a Victorian State Government Operational Infrastructure Support grant.